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Antibodies bound to the blocking peptide no more bind towards the epitope about the target protein. By comparing the staining with the blocked antibody compared to the antibody by itself, one can see which staining is particular.
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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by the two antibodies...
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Blocking peptides are peptides that bind specifically for the goal antibody and block antibody binding. These peptide typically has the epitope identified via the antibody.
Target Data Cyclin E belongs on the really conserved cyclin relatives, whose customers are characterised by a remarkable periodicity in protein abundance from the cell cycle. Cyclins perform as regulators of CDK kinases. Distinct cyclins exhibit distinct expression and degradation patterns which lead on the temporal coordination of each and every mitotic party. Cyclin E sorts a complex with and features for a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates with the G1-S stage boundary and is degraded as cells progress as a result of S stage.
Overexpression of this gene has actually been observed in lots of tumors, which ends up in chromosome instability, and so may add to tumorigenesis. This protein was located to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a important function in promoting cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are already explained. Two additional splice variants were being claimed but in-depth nucleotide sequence details is not really but available.
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